Real-time investigation of protein unfolding at an air–water interface at the 1 s time scale
نویسندگان
چکیده
Protein unfolding at an air-water interface has been demonstrated such that the X-ray reflectivity can be measured with an acquisition time of 1 s using a recently developed simultaneous multiple-angle-wavelength-dispersive X-ray reflectometer. This has enabled the electron density profile of the adsorbed protein molecules to be obtained in real time. A globular protein, lysozyme, adsorbed at the air-water interface is found to unfold into a flat shape within 1 s.
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عنوان ژورنال:
دوره 20 شماره
صفحات -
تاریخ انتشار 2013